This course will first consider the mechanisms and kinetics of hydrolytic enzymes. Methods for protein analysis and purification will then be described, leading to a discussion of the use of recombinant DNA technology in the large scale production of proteins in the laboratory. The second part of the course involves study of the importance of lipids, carbohydrates and hormones in health and the diagnosis of disease. The use of antigens and metabolites as markers of disease will also be discussed.
Learning outcomes:
At the end of this module students will be able to:
1. describe methods for protein purification;
2. describe the turnover of enzymes both mechanistically and kinetically;
3. perform calculations to quantify enzymatic activities;
4. describe the clinical importance of the metabolism of calcium and lipids;
5. discuss the functions of the liver and kidney and describe the biology of diseases that affect these organs;
6. discuss the use of therapeutic drug monitoring in Biomedical Science;
7. perform biochemical assays in the laboratory.
- Module Supervisor: Paul Dobbin